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mouse brain total cdna  (TaKaRa)


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    TaKaRa mouse brain total cdna
    Mouse Brain Total Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+brain+total+cdna/pm38170683-47-19-23?v=TaKaRa
    Average 93 stars, based on 497 article reviews
    mouse brain total cdna - by Bioz Stars, 2026-08
    93/100 stars

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    Ion channel genes ( n = 42) profiled by qPCR with single-cell cDNA library prepared from MVN neurons ( n = 167)

    Journal: The Journal of Neuroscience

    Article Title: Graded Coexpression of Ion Channel, Neurofilament, and Synaptic Genes in Fast-Spiking Vestibular Nucleus Neurons

    doi: 10.1523/JNEUROSCI.1500-19.2019

    Figure Lengend Snippet: Ion channel genes ( n = 42) profiled by qPCR with single-cell cDNA library prepared from MVN neurons ( n = 167)

    Article Snippet: For 37 of 55 genes whose transcript levels are reported in this paper, the amplification rates were empirically measured by qPCR with a serial dilution series of cDNA prepared from mouse brain total RNA (Zyagen).

    Techniques: cDNA Library Assay, Expressing

    Genome-wide screening of genes that are coexpressed with fast-spiking ion channel genes in a graded manner. A, Dendrogram showing expression correlation of genes in MVN neurons. Gene expression was quantified by microarray using a pooled cDNA from each cell type. The branch indicated in red includes all of the ion channels that showed highly correlated expression with maximal firing rates of MVN neurons. Note that the microarray failed to detect Cacna1a even though its high expression was confirmed by qPCR (Fig. 4B, right). B, Transcript levels of neurofilaments plotted against Kcna1. Symbols in B indicate MVN cell types as shown in the legend on the right. C, Transcript levels of synaptic molecules (Vamp1, Cplx1, Syt2) plotted against Kcnc1. D, Transcript levels of Scn4b against Kcnc1. E, Transcript levels of representative genes that did not show positive correlation with fast-spiking ion channel genes, plotted against Kcnc1. For specifics of cell classification, see Figure 5-1, and for the list of probe sets included in the red branch of the dendogram in Figure 5A, see Figure 5-2.

    Journal: The Journal of Neuroscience

    Article Title: Graded Coexpression of Ion Channel, Neurofilament, and Synaptic Genes in Fast-Spiking Vestibular Nucleus Neurons

    doi: 10.1523/JNEUROSCI.1500-19.2019

    Figure Lengend Snippet: Genome-wide screening of genes that are coexpressed with fast-spiking ion channel genes in a graded manner. A, Dendrogram showing expression correlation of genes in MVN neurons. Gene expression was quantified by microarray using a pooled cDNA from each cell type. The branch indicated in red includes all of the ion channels that showed highly correlated expression with maximal firing rates of MVN neurons. Note that the microarray failed to detect Cacna1a even though its high expression was confirmed by qPCR (Fig. 4B, right). B, Transcript levels of neurofilaments plotted against Kcna1. Symbols in B indicate MVN cell types as shown in the legend on the right. C, Transcript levels of synaptic molecules (Vamp1, Cplx1, Syt2) plotted against Kcnc1. D, Transcript levels of Scn4b against Kcnc1. E, Transcript levels of representative genes that did not show positive correlation with fast-spiking ion channel genes, plotted against Kcnc1. For specifics of cell classification, see Figure 5-1, and for the list of probe sets included in the red branch of the dendogram in Figure 5A, see Figure 5-2.

    Article Snippet: For 37 of 55 genes whose transcript levels are reported in this paper, the amplification rates were empirically measured by qPCR with a serial dilution series of cDNA prepared from mouse brain total RNA (Zyagen).

    Techniques: Genome Wide, Expressing, Gene Expression, Microarray